Principles of Electron Microscope
An electron microscope utilizes the signs that arise from the contact of the electron with the model to get relevant knowledge about the construction and its work. It consists of an electron gun that helps to generate the electrons. In addition to this, it also carries two pairs of condenser lenses that focus the beam of electron on the sample, and after that, it turns into a thin light beam. The sample is to examine and also it is made up of thin, which is at the most 200 times thinner compared to that of an optical microscope. The electron beam that passes through the specimen is sprinkled based on the width or the refractive index of several elements of the samples. An electron microscope uses the signals from the interaction of the beam of an electron with the sample to gather the details of the sample. The electron ray that arises from the samples travels through the objective lens, has an elevated influence, and shapes the exaggerated transitional image.
Components of Electron Microscope
An electron microscope carries a higher range of magnification which can be helpful to detect smaller structures. It also has a higher resolution that can provide a clearer and also more thorough image. It can also be used to analyze the structures which have been able to get a detailed idea of light microscopy. It has a diverse application, and it can be used with a wide series of purposes in various fields of research that include technology, industry, and biomedical science. With respect to appropriate training, an electron microscope can use the proper classification parts to create a high image of a good quality structure.
The electron microscope also carries some limitations. The live specimen cannot be observed. The object has to be ultrasound as the penetrating power is very low. Due to this, the samples have been dried and also cut into ultra-thin parts before the observation. It is very expensive to build and also to maintain the electron microscope. The image artifacts and resulted in different samples training. This kind of microscope is huge and extremely very responsive to trembling and exterior magnetic fields. As other different molecules sprinkle the electrons in the air, the samples should be analyzed and viewed in a vacuum. As the electron microscope works in a vacuum, the samples are therefore completely very dried, and an electron microscope can only reveal black and white images.
Types of Electron Microscope
Based on the functioning of the microscope, the electron microscopes can be classified into two major types, which are as follows: There are two types of electron microscopes based on the type of function performed by them.
Transmission Electron Microscope or TEM:
It is used to examine thin specimens that allow electrons to flow through and create a projected picture.
In many respects, the TEM is similar to a traditional compound light microscope.
The major applications of TEM include examining the minute details of the cell organelles in thin sections, detailing the structure of protein molecules, detecting the cellular organization of the viral molecules, sketching the cytoskeletal microfilaments through negatively stained sections, and determining the arrangement of proteins in cell membranes through freeze-fracture techniques.
Scanning Electron Microscope or SEM:
Conventional SEM is based on the scanning of the secondary electrons emitted from the surface of the object or specimen.
SEM is equivalent to a stereo light microscope due to its high depth of focus.
It can provide detailed pictures of the surfaces of cells and entire organisms that TEM cannot focus. It may also be utilized for counting the particles, determining their size, and controlling processes.
The image is produced by scanning a focused electron beam onto the surface of an object in a raster pattern, hence the name “scanning electron microscope.”
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